Innocent Damudu Peter

10 Questions 38 Answers 0 Followers

Questions related from Innocent Damudu Peter

Are there differences between steroid hormone levels when extracted from fresh or from dried feces?

I have read some few articles that have extracted steroid hormone and their metabolites from feces of animals. Is there any literature that discussed this matter ?

05 February 2019 1,392 7 View

Why is it necessary to add water in the extraction of progesterone metabolites fro oven dried fecal samples?

In the analysis of fecal samples, oven dried fecal samples are commonly mixed with distilled water and diethyl ether for the extraction of progesterone metabolites. Diethyl ether is understood to...

21 November 2018 2,687 0 View

Does anyone has a simple protocol for preparing pregnanolone hemisuccinate ?

Preganolone needs to be conjugated to a protein carrier to mount an immune response. Does anyone have a very simple method of preparing pregnanolone hemisuccinate before conjugation to BSA

19 November 2018 6,861 3 View

Can antibodies be produced against an antigen that has a molecular weight of 318.49 while conjugated with Freunds adjuvant and given booster inj.?

I want to challenge rabbits with an antigen having a molecular weight of 318.49 to harvest antibodies. is it possible for antibodies to be produced against this antigen after mixing it with...

10 November 2017 2,489 3 View

Does anybody has an idea as to how long can antibodies to steroid metabolites (for example Progesterone) remain stable ?

Some antibodies survive are know to be stable at +4, others at -20 and even -80 degrees according to some literature (Ritter, Methods in Molecular Medicine). What then is the safe temperature and...

10 January 2017 3,877 4 View

I need to be very sure on this. Are all fecal progesterone metabolites insensitive to light after methanol extraction?

is there need to use brown (for light sensitive) HPLC vials for separation of fecal progesterone metabolite using HPLC or i can still proceed with the plain vials?

06 October 2016 5,937 2 View

Does anybody have an idea on the molecular wieght of antibodies to fecal progesterone metabolites (5 alpha and beta series)?

Molecular wieght of proteins and associated materials can be used for qaalitative analysis using western blot etc. i need to find the properties of fecal progesterone metabolites

27 September 2016 2,449 4 View

Besides progesterone metabolites and Aglaia perviridis are there other sources of pregnanes?

before now, i thought pregnanes were only found as by products of progesterone metabolism by the liver. i just went through an article " Two new pregnanes from Aglaia perviridis Hiern" by Yang et...

30 August 2016 8,452 0 View

Besides hormone analysis, is there any simple and accurate method of determining the stages of oestrus in cattle?

estrus is clearly expressed by physical activity, what of diestrus, metestrus

26 July 2016 3,401 14 View

Besides use of ethanol, is there any other alternative medium to which fecal samples can be stored before transport to the laboratory for analysis?

Besides use of ethanol, is there any other alternative medium to which fecal samples can be stored before transport to the laboratory for analysis? transport medium for fecal samples for stability...

17 July 2016 9,302 1 View