mostly people observe 1D HOE for 1H-19F or 1H-31P.
What about 1H-13C?
I am trying to eliminate infection from my plates and therefore, gave UV treatment after addition of amino acid (used for better growth). Can you please suggest if UV destroys amino acids or not,...
02 July 2024 2,922 1 View
What are the key challenges in implementing cross-border agreements for sustainable resource management between Nepal and India, specifically related to the extraction of river resources, and how...
25 June 2024 2,462 0 View
I have having issue with my DLS results
25 June 2024 2,218 1 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
Hii experts , i have synthesized a derivativ of biopolmer and zeta potential is a -1.2 mv,,can u tell me how it help me out to study my synthesized product ?
05 June 2024 4,720 3 View
Hi, I have been trying to extract Plant genomic DNA via CTAB method and I am getting a 260/280 ratio ranging between 1.3 to 1.6. Any idea how to modify the protocol for fabaceae family plant...
03 June 2024 7,722 6 View
Hi, I have few primers that are previously reported to amplify a conserved region. The ambiguity is that I want to be sure if they are correctly reported before ordering the same set of primers....
03 June 2024 4,167 3 View
In the context of increasing global water scarcity, why is the delineation of groundwater resources vital for a country's water security, economic stability, and ecological sustainability, and...
15 May 2024 3,868 0 View
Thermal water has Na-119 ppm Cl-469 ppm and SO4 -406 But it is not located anywhere near seawater area
01 May 2024 3,836 5 View
Is there a formula that I can use to calculate the power output using solar irradiance values?
01 May 2024 8,233 3 View
I have done NMR analysis of exopolysaccharide samples. I have to identify the monosaccharide composition and the functional groups the NMR peaks corresponds to. Since, my EPS is unknown i am...
15 May 2024 7,631 1 View
I am expressing a membrane protein (about 15 kDa) in BL21(DE3) cells. The expression is good, and the yield is decent, but each time I checked the mass with a TOF mass spec, the mass I get is...
11 April 2024 7,169 3 View
I synthesized some 1,2,4-triazoles and the two quaternary carbons of the 1,2,4-triazole ring were appeared as very low intensity (even broad) peaks in 13C NMR spectra. This observation is with the...
06 September 2023 2,157 3 View
How can we employ NMR spectroscopy to determine the molecular weight of polyethylene glycol (PEG)? Is it feasible to use NMR to quantify the number of monomers within the PEG chain?
30 June 2023 2,561 7 View
Hello, I came across this paper: Production of isotopically enriched high molecular weight hyaluronic acid and characterization by solid-state NMR, available on researchgate in preprint form....
11 June 2023 2,621 0 View
Dear all, Here I noticed a peak signal of CO2 in Raman spectrum after increasing accumulation time, between the two peaks of 1280 cm-1 and 1380 cm-1, (as shown in the attached figure) which are...
17 May 2023 9,065 0 View
How long will it take to do solid state 1H and 13C NMR of supramolecular structure like MOFS, COFS and self assembly molecuels. Is single crystal solid state NMR possible? Thank you
22 February 2023 7,215 1 View
I'm confused! In 13CNMR spectroscopy, is 13C nucleus used in the device? What exactly is the nature of the magnetic field produced by the device? From carbon 12 or 13? And does it only stimulate...
28 January 2023 9,030 1 View
Hi everyone, My lab is trying to develop a method to quantify water soluble vitamins (more specific, biotin and B12) in LC-MS. However, the vitamin B12 13C standard commercially available is just...
08 December 2022 4,319 6 View
I am beginner in the nmr spectroscopy can anyone help me how to interpret 2d analysis and structure elucidation. analysis type cosy HMQC HMBC NOESY NOE ROSY ETC
21 November 2022 4,329 2 View
