isolation criteria
suitable media
preservation during transportation
incubation temperatures
About preservation during transportation, in my experience, I don't think you'll need any special treatment. Just take the sample as much as possible and put it on an sterile container. And you might want to inoculate the bacteria directly from your hot spring/ land fill site in the enrichment medium such as Luria Bertani or Nutrient Broth.
Regarding the incubation temperature, I would recommend to measure the temperature in the hot spring as well as in the land fill site. Therefore you could use those temperature as a standard. However, usually you can use 37 C for soil bacteria isolation.
For suitable media, sometimes, there're some specific compound from it's origin which is needed for bacterial growth. So, you can use the sample itself to provide those nutrients (example, water from hot spring and soil from land fill). Dissolve general medium such as LB or NB using the water from the hot spring and sterilize it using autoclave as usual. If you want to isolate bacteria from soil, you can dissolve about 10 g of soil in 100 ml of distilled water and use the water to dissolve the general medium.
I hope this would help.
Take sample and preserve in an asceptic conditiona and keep in low temperature.Follow any text book or published method for new bacteria isolation.Thanks
http://mic.sgmjournals.org/content/9/3/485.full.pdf
http://aem.asm.org/content/53/5/942.full.pdf
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