Christine Dwi A P W

9 Questions 10 Answers 0 Followers

Questions related from Christine Dwi A P W

Is it possible to construct a PCR primer based on the EST Clone data?

From the Microarray data (based on the EST clone), I found several genes that needs deeper research to find out the gene expression under heavy metal stress. Thus, we need to conduct an qPCR to...

29 July 2016 302 3 View

Why organic mercury compound is more dangerous than inorganic mercury compound?

I knew that inorganic mercury compound such as mercury chloride or mercury iodide is toxic. Organic mercury compound such as methyl mercury or dimethyl mercury are more dangerous. From what I have...

01 April 2015 1,908 14 View

Why did streak plate method failed to isolate bacteria?

I have done 2 different method to isolate mercury resistant bacteria from Soil. 1. Streak plate method: From my mix culture in Nutrient Broth, I got 28 colonies. Those 28 colonies were subcultured...

12 March 2015 8,404 5 View

Can anyone suggest some DNA plasmid isolation protocols?

I'm going to isolate plasmid from unknown soil bacteria, is there any simple plasmid isolation protocols? Since using a kit is actually my last option, so I'm trying to use manual protocols...

01 September 2014 2,100 2 View

Do all of Mer genes (in all of bacteria) have some kind of similar sequence?

Is there any similarity between Mer genes sequence in all bacteria? i'm trying to isolate mer genes from unknown bacteria from gold mining site in Yogyakarta, Indonesia. If all of the mer genes...

15 August 2014 4,490 2 View

What caused the foggy area that formed in the x-ray film due to bacterial mercury volatilization? is it mercury chloride or Hg0?

in Simplified X-ray film method for detection of bacterial volatilization of mercury chloride by Escherichia coli, When i read the abstract, i thought it was about Hg0 detection using x-ray film....

05 August 2014 3,090 5 View

What's the point on converting Hg2+ to Hg0?

There're so many publication about mercury bioremediation by using mercury reducing bacteria that reduce Hg2+ to Hg0. But Hg0 is actually pretty dangerous as well as Hg2+. So why is converting...

17 June 2014 9,208 5 View

Can anyone help with mercury (0)/ Hg0 detection assay?

I'm using mercury reducing bacteria. I want to know if they're really reducing Hg2+ to Hg0. So far I got Hg0 detection assay using x-ray film. But maybe there's a simpler way rather than using...

17 June 2014 3,792 3 View

Does Pseudomonas putida KT2440 only accumulate Cadmium or is there any reduction/biomineralization mechanism?

Pseudomonas putida KT2440 could accumulate cadmium using protein transporters such as CadA, and CaDB. But after exporting the Cadmium inside, how do they maintain other metabolism and not become...

06 May 2014 7,914 1 View