Can someone suggest the best ECL substrate kit which is sensitive as well as cheap?

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Alejandro Martin

Take a look here: https://www.google.com/search?q=homemade+ECL+reagents&hl=en

David Aguado-Llera

Santa Cruz Biotechnology used to have an ECL kit somehow cheap, but it only worked properly with their specific antibodies. Maybe you have to check for "homemade" ECL reagents or try the kit from your antibody supplier...

Katerina Rohlenova

I would recommend Luminata Forte substrate from Millipore. It is premixed and it also works well when you strip the membrane for incubation with multiple antibodies. Price is reasonable, however you may searched for something much cheaper...

http://www.millipore.com/catalogue/item/wbluf0100