Can someone suggest possible loophole why PMA differentiated THP-1 cells are non responsive to LPS (sigma)?

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Jai Ghosh

I am attaching an article for you to read and see it might answer your question.

Shen-An Hwang

What are you doing to measure response? And what LPS you are using? They do respond to LPS, even at 10ng/mL.

Kuldeep Nigam

thank you for your suggestions. I am using LPS (Sigma) and wish to quantitate NO levels after addition of drugs.

I mean LPS from what strain of e.coli. Some works better than others. I think the strongest LPS is from O111:B4.

This might help. There was another discussion similar to this on another thread. Take a look at their suggestions.

https://www.researchgate.net/post/Does_anyone_stimulate_THP-1_with_LPS_to_detect_IL-1beta_and_IL-12_in_supernatant

Thanks Shen-An for your response.