Kuldeep Nigam

4 Questions 10 Answers 0 Followers

Questions related from Kuldeep Nigam

Can someone provide the list of cell lines available at NCCS, Pune, India?

I need to purchase a few cell lines but I am not sure whether those are available with them or not.

01 January 2019 3,620 7 View

Can someone suggest some brain endothelial cell lines?

Preferably Biosafety level 1. Also, if you can tell from where we can procure them in India.

12 December 2018 8,596 6 View

Can someone suggest possible loophole why PMA differentiated THP-1 cells are non responsive to LPS (sigma)?

I have analyzed at different concentrations of LPS from 10ng/ml - 1.5ug/ml but there is no significant role of LPS observed. Are these cells LPS tolrent?

19 July 2018 2,883 5 View

Can someone suggest a method for revival of THP-1 cell line?

Cells are healthy when used routinely but once freeze downs are made (FBS + 10%DMSO) and stored in -80 C their revival is quite difficult and majority cells are dead. I am using RPMI-1640 media...

16 June 2018 6,130 3 View