Can someone suggest a good fluorescent dye for the detection of alkyne containing proteins? The protein is not soluble so it would need to be detected after denaturation / separation in SDS-PAGE, and after transfer (or by in-gel labeling if that’s doable). Currently I’m thinking Alexa Fluor 488-azide would be the best choice, but would like to hear what your experiences are with the labeling.

All suggestions are much appreciated!