Can someone suggest a flow cytometry based method to assess the viability/sterility of pollen grains using propidium iodide?

More Himanshu Tillu's questions See All

What is the difference between the rotational velocity and cmrotate command in ansys?

I am trying the simulate the drum brake squeal in ansys. For giving the rotational velocity to drum what should i use the Rotational velocity or CMROTATE command.

24 June 2024 5,125 0 View

Next APDL command to get eigenvalues?

I need to get the eigenvalues after a modal analysis in ansys workbench. Is there any next APDL command that will get the required result.

20 June 2024 1,668 0 View

How to create Component Model Synthesis in Ansys Workbench?

I have an assembly of parts. I want to reduce the number of modes of the individual parts by CMS.

11 June 2024 2,966 0 View

I am trying to do LCA of Water Treatment Technologies. Are there any recommended introductory resources or textbooks on LCA that you suggest?

Some Other Questions, if you can answer: What types of data are essential for conducting an LCA, and where can I find reliable data sources for water treatment technologies? How do you deal with...

11 June 2024 4,791 0 View

How to FE model from Modal analysis to use in Static Structural in Ansys?

I have two components for which I want to extract some number of modes shapes for each. Then using those model of the component I want to couple them together for non linear static analysis.

10 June 2024 1,234 0 View

How to create solid45 elements to an existing cad geometry?

I imported an 3D cad geometry in ansys. Now I want to create the mesh with solid45 element in workbench. How to proceed?

05 June 2024 2,490 1 View

Among Ansys and Abaqus which one is preffered for Modal analysis?

I want to do modal analysis on a body which has two components which are in dynamic elastic and frictional contact with each other.

04 June 2024 8,493 6 View

How can we reconcile the apparent contradiction between the statement "All productive soil are fertile" and "all fertile soil are not productive"?

19 April 2024 3,312 1 View

How to do the transfection by lipofectamine method? at what ration we will add lipo:dna?

lipofectamine method for the transfection in MDA-MB231 cell line

18 October 2023 3,311 0 View

How to claim authorship of article?

how to claim authorship of article?

08 September 2023 6,870 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

What is the best method to detect the chemical composition of pollen ?

I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?

28 July 2024 5,331 1 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View