Himanshu Tillu

5 Questions 6 Answers 0 Followers

Questions related from Himanshu Tillu

Why are my pollen grains dying in Phosphate Buffered Saline (PBS)?

I have harvested pollen grains from Periwinkle using needle and forceps an resuspended them in PBS/normal saline. Then I assessed their viability using the Sysmex Pollen Viability Kit (Fluorescein...

31 January 2020 2,186 1 View

Can someone suggest a flow cytometry based method to assess the viability/sterility of pollen grains using propidium iodide?

I am looking to assess the viability/sterility of pollen grains for the study of male sterility. I am looking for a flow cytometry based assay, preferably using propidium iodide or any other...

26 February 2019 2,194 3 View

What are the pros an Cons of VenturiOne flow cytometry data analysis software compared to FCSExpress, FlowJo and Kaluza?

I have used FCSExpress extensively and I like it. I want to know the strengths and weaknesses of other analysis softwares primarily VenturiOne, FlowJo and Kaluza.

14 February 2019 6,167 0 View

Is there a Fluorescence FCM based method to assess pollen sterility (not viability)?

Dear Researchers, I am seeking a method to assess the sterility (not viability) of pollen grains using fluorescence flow cytometry. I have gone through the relevant literature. The best match is...

31 July 2018 1,721 5 View

Why am I getting a precipitate after adding 1:1 diluted Folin's reagent?

I have expressed a viral protein in E.coli. and purified it using Ni affinity chromatography. I have refolded it in vitro and dialysed it into PBS. I then performed protein estimation by Lowry's...

07 October 2016 4,997 3 View