FAME profiling of bacterial cell wall hydrolysate is one of the identification tool for bacterias. Can anyone share the full protocol to extract, prepare and identify the FAME using GC-FID analysis.
Can anybody give a sample file for extracting turbulent energy spectrum from LES simulation in OpenFOAM. I am struggling to get it. Thanks in advance.
24 May 2022 6,745 0 View
An axially loaded member is modeled as depicted in the figure attached. As we keep refining the mesh, the stress near the node where load is applied keeps on increasing, which indicates that...
01 September 2020 5,627 3 View
Experts could suggest me some tips to improve my communication to the supervisor.
14 December 2017 7,959 3 View
How do you detect the base peak and the fragment peaks of a purified single molecule? Likewise how do you detect the base peak and the fragment peaks of a crude extract?
27 February 2014 2,915 15 View
Can someone share the details of number of secondary metabolite produced by a bacteria. The number may vary from species to species. Is there any references to address this question. How to detect...
02 January 2014 8,438 4 View
I would like to know where I can find the exact list of approved antibacterial antibiotics. If you knows the the way to find this list, please share.
10 August 2013 7,790 4 View
Please discuss the topic. Update: I have few mg of TLC purified compound from a microbial secondary metabolite. With this how can I check in both GC-MS and LC-MS? How can I identify my compound...
07 May 2013 8,883 23 View
1. Crude - Column chromatography - TLC - RP.HPLC 2. Crude - TLC - Column chromatography - RP.HPLC 3. Crude - TLC - RP.HPLC 4. Crude - Column chromatography - Preparative HPLC Can anyone suggest...
10 April 2013 1,101 11 View
I did 16S rRNA sequencing and SEM analysis for the morphological characterization, but I want to know, how can I be certain that this is a new species?.
28 March 2013 7,968 3 View
I have problems in actinomycete culturing, maintaining and during incubation periods. I am using petri plates for the screening of actinomycetes. During the incubation periods in the incubator I...
21 March 2013 3,941 4 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View