Can someone recommend me how to plot such pie chart for analyzing antibody germline usage and somatic hypermutations?

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Charge transfer characteristics of inverted perovskite leds?

When testing devices based on ITO/ZnO/CsPbBr3/TFB/MoO3/Ag structures, I found that the current density of the device always increases sharply to 1000mA/cm2 at about 3V bias, and the device does...

16 July 2024 4,291 0 View

How can I do photodetector application from Inorganic nanoparticles compound?

I have an inorganic nanoparticle compound I want to do a photodetector application from this compound, I do not have any idea, can anyone help me with this?

11 July 2024 135 1 View

The difference between the E. Laviron's model and RICHARD S. Nicholson's model for electrode reaction kinetics?

For the measurement of electrode reaction kinetics and electron transfer rate constant, using E. Laviron's model and RICHARD S. Nicholson's model, the greater peak potential separation indicates...

30 June 2024 580 3 View

Is there any chance that the arc-continent subduction could happen?

With the closure of the back-arc basin, oceanic island arcs can collide with continental margins. Theoretically, is there a set of unique conditions under which, following such a collision, the...

15 June 2024 2,410 0 View

Which Hybrid Sorghum Varieties Are Most Effective in Saudi Arabia for Silage?

Investigating use of Sorghum as part of Regenerative Agriculture management system using cover crops & mower-crimping, Alley Cropping, sheep manure in a zero till management system with...

18 April 2024 2,389 3 View

Does mutation in long terminal repeat (LTR) regions of lentiviral plasmid affect gene delivery?

Hi lentivirus experts, I have lentivirus plasmids that carry mutations in the 3' and 5' LTR region. These regions were not exactly designed for easy cloning. Therefore, I wonder if it is possible...

14 April 2024 3,054 2 View

In HFSS, how to get the Reflection Parameters of a metasurface illuminated by a Circularly Polarized Wave?

I am trying to design a Circularly Polarization reflective metasurface. I want to know , how to Get the accurate circularly polarized reflection parameters when I try to illuminate the unit cell...

05 March 2024 7,917 0 View

What's the different between soluble ova and latex-bed ova in dendritic cells research?

In some research, some researchers used both soluble OVA and latex-bed coated OVA in dendritic cells antigen loading, and they had different effect on T cell stimulation. I would like to know...

14 February 2024 7,102 0 View

How to co-label two proteins using primary antibodies of the same animal (ICC)?

I am doing ICC to check for colocalization of my protein of interest and mitochondria. Without having to use Mitotracker, (I'm currently have issues with finding the optimal concentration) or...

01 February 2024 3,815 1 View

How to extract transform Acrylohydrazide hydrochloride into non-ionic form?

I synthesized a monomer ionic form (Acrylohydrazide hydrochloride), which was dissolved in water. How can i get the non-ionic form (Acrylohydrazide) without other impurities?

21 January 2024 1,923 1 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why applying a saved template of a bar chart in a different/new one doesn't take effect in SPSS?

Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...

29 July 2024 1,981 1 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View