How to solve carry-over and interference problem when detecting tulathromycin by LCMS?

I am working on the determination of tulathromycin in rat faeces using QE-focus (Thermo Fisher)(Waters BEH C18, 2.1x100mm, 1.7um)(0.1%FA+H2O, 0.1%FA+ACN) . I encountered two tricky problems....

02 January 2021 2,713 5 View

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21 October 2020 7,860 3 View

Can anyone share image dataset of food such as cheese, fruits, one object per photo?

I need a dataset of food items. Prefer one object per photo. Any hints are helpful.

05 October 2020 536 5 View

Electropolymeriztaion to synthesis polymer film. But it is too thin, what to do?

Hey guys, these days I am using electropolymerization to form polymer film. I mimiced the procedure of the paper, and got very thin membrane (~hundreds of nanometer), while the paper says they...

24 August 2020 3,379 7 View

What is the average velocity formulae of the moving object in 3D using optic flow?

As i searched in optical flow technique (OF) many theories given where sensing the velocity based on images captured during the motion in 3D but i want the average speed formulae . can anyone who...

11 August 2020 3,970 1 View

Is there a gentle method to reduce cuprous oxide to copper while maintaining the original morphology?

Hello, Recently, I have synthesized some cuprous oxide nanomaterials and want to reduce them to metal while maintaining the morphology. Is there a way to do this?

04 August 2020 4,850 3 View

What is the best way to register crop point clouds and spectral information in a plot of the field ?

Hi everyone, The goal: i want to register crop point clouds and spectral information in a plot of the field. The data detail: 1.The crop point clouds will be obtained by terrestrial Lidar (or UAV...

05 July 2020 9,128 5 View

I got Raman peaks for CsPbBr3 Nanowires respectively at 2450, 3640 and 3870 cm-1. What do they belong to?

The peaks were obtained for CsPbBr3 Nanowires excited by a 532 nm laser. Needs a clear explanation.

15 May 2020 8,103 3 View

The collagen gel evaporate with 3D organoid model (air Liquid interface method), why?

Hi, guys, I have a queation, please help me. I used air liquid interface method to do 3D culture. This is the first time to do it. the collagen gel prepared according to literature, 8 vol of...

13 May 2020 7,288 2 View

How often to get more loci/SNPs from reference based compare to de novo in RADSeq?

Hi, I have this simple but wired question that how many of you ever compared the results of the same data set using both reference-based and de novo approach, and get more loci/SNPs using the...

16 February 2020 9,641 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View