I synthesized a monomer ionic form (Acrylohydrazide hydrochloride), which was dissolved in water. How can i get the non-ionic form (Acrylohydrazide) without other impurities?
you can extract it in DEE before dissolving it in water.
When testing devices based on ITO/ZnO/CsPbBr3/TFB/MoO3/Ag structures, I found that the current density of the device always increases sharply to 1000mA/cm2 at about 3V bias, and the device does...
16 July 2024 4,291 0 View
For the measurement of electrode reaction kinetics and electron transfer rate constant, using E. Laviron's model and RICHARD S. Nicholson's model, the greater peak potential separation indicates...
30 June 2024 580 3 View
LS-DYNA finite element simulation:During the battery impact simulation, some units experienced failure and subsequently underwent penetration. How can this issue be resolved?
24 June 2024 3,917 0 View
Hi everyone! I am using a Q5 high fidelity DNA polymerase to amplify a 6.7 kb plasmid. The PCR with Q5 went well with longer primers (~55 bases) at 70-72 degree C. However, I keep failing in the...
17 June 2024 1,734 4 View
With the closure of the back-arc basin, oceanic island arcs can collide with continental margins. Theoretically, is there a set of unique conditions under which, following such a collision, the...
15 June 2024 2,410 0 View
how do I change my interest in Researchgate? There are many notifications regarding articles, which I don't like at all. The website indicates they are according to my interest, but I can not...
11 June 2024 8,509 2 View
This question addresses the ongoing challenge of improving cancer therapies by focusing on the development of advanced drug delivery systems that can more accurately target cancer cells, thereby...
28 May 2024 6,197 5 View
What should the requirement be for an all-inorganic perovskite solar cell? What should be the nature of its layer? If we use any organic layer in an all-inorganic perovskite active material-based...
24 May 2024 3,355 2 View
who can send it to me?it is important for me.thanks.
14 May 2024 5,983 4 View
As known, the spin exchange coupled Mn4+ leads to a narrowd singal, at ca. g=2.006 with the line width about 500 gauss. Why the birnessite MnO2 dispalys such wide spectrum? Is any other exchange...
23 April 2024 6,351 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...
01 August 2024 8,754 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
Thanks
22 July 2024 1,143 3 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View