I am looking for general MS media recipes. I can find hormone concentrations optimized for different species but I would like something more general purpose that can work for many species and may be optimized later. Can someone recommend MS media hormone concentrations for rooting, shoot multiplication, callus induction, and vegetative growth?
I have BAP, NAA, IBA, IAA, TDZ, GA3, 2,4-D, and kinetin to work with.
Dear Robert Samples
Pls. find the attached files
http://www.biologydiscussion.com/plants/plant-tissue-culture/plant-tissue-culture-media-types-constituents-preparation-and-selection/10656
http://www.caissonlabs.com/us.php?chooseacc=2
https://ar.scribd.com/doc/24201583/Preparation-of-MS-Medium-From-Stock-Solution
https://www.intechopen.com/books/recent-advances-in-plant-in-vitro-culture/plant-tissue-culture-media
http://hos.ufl.edu/sites/default/files/courses/hos6373c/february%205/media2.pdf
http://hos.ufl.edu/sites/default/files/courses/hos6735c/MediaFormulations.pdf
http://www.academia.edu/5082463/Media_Components_and_Preparation
Hoping this will be helpful to your project, good luck
Best regards
Prof. Houda Kawas
In general, all composition was same for ms medium except hormone. Please see the link for better idea about ms media preparation procedure.
http://bioprofessor2020.blogspot.com/2012/02/plant-tissue-culture-medium-m-s-medium.html
For Callus Induction
Carbon resources : 30 g/l sucrose
Media 1 : 1 mg/l BAP + 0.5 g/l kinetin
Media 2 : 1 mg/L IBA + 0.1 mg / l kinetin + 0.1 mg/l 2,4 D
Media 3 : 2 mg/l NAA + 0.04 mg/l 2,4 D + 0.4 mg/l MgCl2
and you are also combine 2,4 D + Kinetin + IBA different amount (0.1 to 1 mg/l)
Robert Samples
Murashige and Skoog's media composition of macro, micronutrients, and vitamins. Hormones are not a part of MS medium. Even carbon source and gelling agent have to be added separately. You have to add the growth regulators according to your needs.
In general, cytokinins are required for culture induction, a combination of cytokinins and auxins for multiplication, auxins for rooting, 2,4-D to induce callus, etc. Proportion and types of growth regulators use to change.
Before starting your research, go through basic steps, plenty of information is available online.
Please go through the original article of MS medium
Article A Revised Medium for Rapid Growth and Bio Assays with Tobacc...
