Can someone recommend a reliable but cost-effective plate reader?

More Laura M. McLane's questions See All

Does anyone have an opinion on the BD Accuri C6 Plus Cytometer?

I'm trying to find a simple table top cytometer to do some basic flow cytometery. Many of the models are out of my current budget. Does anyone have any experience with the Accuri C6 cytometer?

10 November 2018 8,993 3 View

Can anyone recommend a good system to look at protein-DNA interactions that does not involve doing an EMSA?

I want to look at putative DNA binding mutants in my favorite protein but am trying to avoid doing EMSAs. Does anyone have a solid assay that they have used to look and protein-DNA interactions? I...

04 May 2014 1,509 9 View

Does anyone know what this nuclear structure is?

I created a GFP-fused mutant of my favorite transcription factor and expressed it in 293T cells. The mutation is in the putative DNA binding domain. It is not co-localizing with regions of dense...

02 March 2014 7,832 5 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

C3D file, how to extract a vídeo file from it?

I made a series of jump tests in subjects, everything was recorded with Vicon. I have data from markers and force plate. We recorded the tests before and after an intervention. My question is, I...

23 June 2024 9,381 4 View

How do I prepare rat cardiomyocyte cell lysate for ELISA via freeze-thaw method?

Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?

23 June 2024 2,264 4 View

What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Different interpolated concentrations?

I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...

15 June 2024 5,109 1 View

Luciferase assay lysis?

Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...

13 June 2024 9,936 5 View

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View

Vikas Taank

Have you considered Tecan Pro 200?

Danielle Dillon

Hi Laura,

Biotek offers several plate readers at different price points (https://www.biotek.com/products/detection/). They are nice and reliable. Personally, I think the software has a bit of a learning curve, but the equipment itself is great.

Ephrem Debebe Zegeye

We use HIDEX sense Microplate Reader. We love it :)

http://hidex.com/products/hidex-sense/

Engelbert Buxbaum

https://www.berthold-bio.com/microplate-readers/, I had good experience with them (for what that is worth)