Can you determine the pH of a solution made up with 65 % Ethanol?

I am performing a test where the pH of the sample needs to be between pH 6 - 8. I combine 10g of dry sample such as ground maize with 50 ml of 65 % Ethanol. The sample is then mixed and the...

21 October 2019 9,657 2 View

How do I make up a 0.01M Na2HP04/NaH2P04 solution?

How do I make up a 0.01M Na2HP04/NaH2P04 buffer solution, pH adjusted to 7? Both chemicals that I have on hand are anhydrous. The molecular weight of Na2HP04 is 141.96 g/mol and the molecular...

10 September 2019 5,524 3 View

Which solvent could dissolve glass fiber, but not carbon fiber. I am looking way sorting the carbon fibers from hybrid mat(Cf/GF) ?

In my research i want to recycle carbon fiber coming from hybrid mat waste. The hybrid woven fabric contains glass fiber yarn and carbon fiber yarn.

10 July 2019 1,716 4 View

How would you test the effectiveness of a preservative in animal feed?

How would you test the effectiveness of a preservative added to animal feed? I've had a look at the preservative efficacy test used for pharmaceutical products: where the preservative is added to...

12 September 2018 6,070 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View