Can someone please share any recent protocol to assay B-1,3- Gluconase enzyme in plants affected with any biotic stresses?

12 December 2017 0 9K Report

If any recent protocol is available to elucidate B-1,3-Gulcanase in plant sample, please share.

Badges
Science topic

More Asish Kumar Panda's questions See All

What is the role of plant endophytes for nutrient absorption and mobilisation?

Endophytes are known to regulate various physiological processes in plants. Plenty number of literature are available supporting their involvement in modulating several signalling pathways with...

02 March 2018 4,747 6 View

How to study the pollen tube growth in a pistil?

I want to know the methodology to study the pollen tube growth in a pistil. Which dye can be used? Can anyone guide me please

31 December 2017 7,203 0 View

Apart from inviting the pollinators, what else is the role of floral nectar in plant reproduction?

Floral nectar is found in many angiosperm. Though it is a rich source of sugar, it contains various amino acids and minerals. I am interested to know the role of nectar for pollination and...

10 November 2017 8,372 5 View

I want to know the procedure to collect the root exudate from banana plant. Can any one please help me??

Root exudate possesses an immense importance for a plant physiologist, microbiologist as well as a pathologist. i want the procedure to extract root exudate from banana.

10 November 2017 866 8 View

What is precision plant breeding?

Precision farming has become popular to everyone associated with advancement in farming strategies. Similarly, in plant improvement what is the scope of precision breeding? I think, it possess...

10 November 2017 6,989 13 View

How can we study the pollen structure?

By using advanced microscopy tools such as SEM and TEM the pollen structure can be studied up to some extent. Is there any other technique available to study the pollen structure of any crop species?

10 November 2017 2,857 3 View

In many cases, increase in ploidy level of a plant causes decreased growth rate. Why?

Increase the ploidy level of a plant may cause higher vegetative growth. Stomatal size as well as pollen size increases with the increase level of ploidy. Morphologically, Leaf area, plant height...

09 October 2017 7,491 8 View

Why success in conventional banana breeding is climate dependant?

Seed set in banana is highly restricted by male and female sterility factors. Even though, male and female fertile parents are used for crossing programme, seed set depends on the climate. How,...

09 October 2017 3,757 3 View

What is climate resilient fruit breeding?

I want to know more about the advance fruit breeding strategies to mitigate the adverse effects of climate changes.

10 November 2016 7,524 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

What is the role of oxidative stress in toxin-induced carcinogenesis?

What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...

02 August 2024 3,174 1 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

Can we eliminate the stress singularity at the tip of the crack by manipulating the elastic constants?

The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.

25 July 2024 9,976 3 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

I have two problems: 1) the enzyme is not immobilizing efficiently into the MOF material.. 2) the MOF itself has peak on 400nm by using p-NPA test.?

I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...

20 July 2024 1,440 3 View