Root exudate possesses an immense importance for a plant physiologist, microbiologist as well as a pathologist. i want the procedure to extract root exudate from banana.
Please find enclosed some links and PDFs...
Technique for collection of root exudates from mature trees,Plant and SoilVol. 32, No. 1 (February 1970), pp. 238-241
Root exudates are usually found in the root plane region of the plant. These are just a few mm from the root. The exudates often serves as a chemotaxic agents for many microbes to come near the root. these may be harmful or useful to the plant. I would recommend that you uproot a banana plant. Gently shake off all the loose mud that may tend to fall off naturally. there will be some mud sticking on the roots still. Now try to scrape off these mud particles which did not fall off easily. These will have the root exudates secreted by the plant. N would need lot of these roots to get a descent amount of extract for your study.
Please find enclosed some links and PDFs...
Technique for collection of root exudates from mature trees,Plant and SoilVol. 32, No. 1 (February 1970), pp. 238-241
Please look for articles published as indicated below:
Methods of collection of plant root exudates in relation to plant metabolism and purpose: A review
Article in Journal of Plant Nutrition and Soil Science 176(2):175-199 · April 2013
J Basic Microbiol. 2012 Oct;52(5):613-5. doi: 10.1002/jobm.201100407. Epub 2011 Dec 5.
A novel technique to collect root exudates from mustard (Brassica juncea).
Wadhwa K1, Narula N.
Kindly use the methodology of Pang et al. 2015 entitled "Physiological and morphological adaptations of herbaceous perennial legumes allow differential access to sources of varyingly soluble phosphate". DOI: 10.1111/ppl.12297
The references and articles which provided by Dr. Anoop Kumar Srivastava and Dr. Ali Alhayany are very important.
Best regards
Dear , Asish Kumar Panda
You can find the following references :
1 - Organic acids from root exudates of banana help root colonization of PGPR strain Bacillus amyloliquefaciens NJN-6. By : Jun Yuan et al . Scientific Reports 5(13438) · August 2015.
2 - The Composition of Root Exudates from Two Different Resistant Peanut Cultivars and Their Effects on the Growth of Soil-Borne Pathogen.
By : Xiao-gang Li et al. Int J Biol Sci 2013; 9(2):164-173 .
Best Greetings
Dear , Asish Kumar Panda ..
Also you can see the following articles , I hope they may be helpful.
1 - Organic Acids, Sugars, and l-Tryptophane in Exudates of Vegetables Growing on Stonewool and Their Effects on Activities of Rhizosphere Bacteria . By : Faina Kamilova et al. Moleular Plant - Microbe Interaction. March 2006, Volume 19, Number 3 Pages 250-256 .
2 - https://www.bioversityinternational.org/uploads/tx_news/InfoMusa_the_international_journal_on_banana_and_plantain_1378.pdf.
Good luck
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts