Can someone please help with my cell seeding calculation?

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Cell culture model of Acute kidney injury?

Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...

29 July 2024 5,622 3 View

Assistance with high taxonomic level identification of intertidal microalgae in SEM Images?

I am currently conducting research on benthic microalgae in the intertidal zone along the central California coast, specifically focusing on grazing by marine snails. As part of this study, I have...

08 July 2024 3,342 5 View

To what extent should "reading comprehension" be analyzed in an A.R. based on phonological awareness instruction to EFL students using a webtool?

Dear Colleagues and academy, I am conducting an action research study to understand the impact of phonological awareness activities using Quizlet and Boom on the reading comprehension skills of...

02 July 2024 8,480 0 View

Does anyone have experience with AML/T cell cultures in serum-free medium?

We want to keep AML cell lines together with T cells in serum free medium for about 4-7 days. Currently we culture them in RPMI 1640 with 10% FCS, so something resembling that would be good I...

22 May 2024 1,763 0 View

What are the mechanisms involved in which bacteria gains resistance against antibiotics?

An important factor to consider when providing antibiotics is the possibility of the bacteria gaining resistance to it. My questions pertains to how these bacteria are able to gain resistance and...

06 February 2024 5,153 2 View

Do you have an opinion on the HI-98194 Multiparameter Waterproof Meter by Hanna Instruments?

longevity, solidity, patency, reliability.... Thanks

24 January 2024 8,325 0 View

To what extent does the use of Quizlet during the pre-reading stage support 4th graders’ ability when reading for gist?

Hello, I am attempting to conduct action research based on the following research question. Could you please provide any advice, authors or research field to deeper exploring? Thanks in advance....

25 December 2023 7,097 8 View

Dear all, how do I access the QA metadata for MOD13A2 composites?

I'm trying to obtain the the quality assurance metadata for MOD13A2 MODIS 16-day composites. Any help will be greatly appreciated.

24 October 2023 353 0 View

Was the king in Ancient Egypt subject to judgment after death?

James Allen (and others) just assert this, but Brandon in the 1950's was not sure, and it does not seem so clear to me either. The Instruction of Merykare seems rather clear that there is a...

03 October 2023 8,463 2 View

How can I prevent cannula exposure of Alzet osmotic pumps?

I am working to establish a protocol for ICV drug administration via an Alzet osmotic pump brain infusion kit using mice. Although, this is a well-established technique, I continue to have issues...

06 July 2023 8,300 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View