Alexis Neumann

9 Questions 9 Answers 0 Followers

Questions related from Alexis Neumann

How do I use the UCSC website to find the promoter region of a gene?

Could someone please explain (step-wise if possible) how I find the promoter region of a gene using the UCSC website. I am a bit lost. TIA

02 February 2017 1,620 2 View

Is this mycoplasma?

Dear Scientific Community :) A colleague recently tested his melanoma (ME1402) cells for mycoplasma and obtained some strange images of rod-like structures - quite different from the 'dot-like'...

15 December 2016 4,701 3 View

Can someone please help with my cell seeding calculation?

I need someone to please double check my calculation. So I need to seed 3.8x10^5 cells per well. Each well takes 500ul and I have 13 wells (hence a total of 6500ul). My cell count is 8.1x10^5/ml....

09 November 2016 4,705 2 View

Can you TA clone an insert into pGEM T easy after designing primers with restriction sites?

Good day fellow researchers, I have recently designed primers with restriction sites which I am going to use to amplify my insert and clone into a reporter vector. After reading up on TA cloning I...

07 November 2016 3,888 10 View

How can I quantify cell colonies using Imagej?

Hi all. I would like to quantify my clonogenic assays using ImageJ. Anyone able to guide me on how I could go about doing this? TIA

04 February 2016 1,362 5 View

Does anyone have advice on how to optimally culture and maintain leukemia cells in suspension?

Good day all, I am currently trying to familiarize myself with the culturing and maintenance of HL60 & JURKAT leukemia suspension cultures. What density should these cells be cultured at, how...

25 January 2016 4,397 3 View

What is the best method for a clonogenic assay for cells in suspension?

I recently started working with suspension cultures (HL60 and JURKATS). I am treating them with a new compound. I have performed clonogenic assays for adherent cells before but never for cells in...

20 October 2015 468 4 View

Could anyone suggest techniques / assays for testing for 2 of the Hallmarks of cancer?

Could anyone suggest techniques for testing the following 2 Hallmarks of Cancer: 1. Resisting Cell Death & 2. Activating Invasion and metastasis? Any literature on this would be appreciated as...

10 November 2014 2,889 13 View

Is anyone able to share melanoma disease progression literature?

I am doing a study on the protein expression profile of melanoma progression (RGP-->VGP-->metastatic melanoma) and looking out for any literature on the molecular changes (at DNA &...

22 July 2014 392 3 View