It is happening that silk worm are not surviving in later stage due to disease, to find out the causal agent we need to measure initial germ load when to start the rearing compare with increasing germ load of a rearing house.
Hi, maybe you should sample micro-biologically from different positions and materials which are involved in rearing like air, water, walls, workers, etc. to determine germ load at the first, the middle, and the end of process. If I understand the question correctly or not, please alert me.
Hi, thank you for your response. Actually we are doing Silkworm rearing in a room where some of the germ causing diseases in the silkworm may be present in the room due to infection of previous rearing of silkworm. I want to determine germ load at the first, the middle, and the end of process.whet is the procedure. I hint it that I can place the uncontaminated medium in the rearing room after disinfection, if the medium is contaminated we can measure it. Or we can put the pathogen it self, if the same is destroyed by the disinfection , it will also serve the purpose. Can you suggest on it.
Hello again. Thank you for explaining your idea. Unfortunately, I didn't have any experience about silk worm pathogens. I hope somebody who has ample information about this matter, help you soon. But the way hit my thought is the work must completely perform aseptically. If it is possible, change the house and use new one. Or disinfect it perfectly and thoroughly and use neat materials which are in contact with worms. About experiments, as you said you can use sterile microbiological mediums to detect germs. One way is as follow: place several plates of medium without their lids at different points of the house for a certain period, for example 10, 20, 30, 60 minutes. Then put their lids and transfer the plates to a microbiological laboratory quickly for further examinations. The other ways as you pointed out are sampling after disinfection. You can use sterile swabs and drag them over places which usually be disinfected hardly like junctions between walls and the location of keeping of silk worms, and so on. Then transfer the swabs into liquid mediums and send them to laboratory. Good luck
I am not sure what is already available or what is feasible, but what would be useful here and in many other situations is for a fluorescent or colour marker to be developed for DNA or other component of germs, so a single picture or photo of the room would quickly and simply give you an overall view.
Thank u R Yalfani to follow the point and of course your suggestion
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