I checked my insert and plasmid. Transformation is working fine. I am getting approximately 25-30 colonies but none turn out to be positive.
Meaning of the term STATISTICS as I have thought of is as follows: S T A T I S T I C S Systematic Tentative Analytical...
28 June 2024 4,011 1 View
Can I use the FA map generated from the Siemens scanner in FSL TBSS? Features Measurement of up to 256 directions of diffusion-weighting with up to 16 different b-values. (up to 192 directions...
11 April 2024 957 3 View
Many research articles, earning positive feedback by researcher(s), have been observed to be hardly cited. This is a point which is to be thought of deeply. I think, in order to cite an...
28 February 2024 9,667 7 View
From my realization, I like to propose the following meaning/interpretation/significance of the term CHRISTMAS 'C' for Cordiality, 'H' for Humanity, 'R' for Rightfulness, 'I' for...
24 December 2023 9,828 9 View
I am adding enough adenine in my plates
06 October 2023 364 5 View
I've a nanomaterial dispersion in water. I want to recover the material in solid form. I have used freeze drying method earlier. Now I want to utilise another method? What would be a facile method...
14 September 2023 9,453 2 View
The normal probability distribution distribution, also known as Gauassian distribution since it is discovered by Gauss, is a symmetric probability distribution. Thus, the skewness of normal...
28 June 2023 6,512 8 View
I've doped a nanomaterial with an electron donor, the X-band ESR spectra does indicate that there is a change in the line widths of both the spectra along with a slight change in the g-factor...
07 May 2023 3,595 2 View
Let's say I've a host-guest complex. I've two sets of Uv-Vis data, one where the host concentration is constant and the guest is varied and in the other the guest's concentration is constant and...
26 April 2023 1,236 2 View
Suppose, we have a number of sets of data and we want to compare the variations of the sets. We know that coefficient of variation (CV) is the measure which is used and/or can be used in this...
18 December 2022 3,153 9 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,572 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,056 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,986 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,891 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,647 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,228 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,556 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View