What are the methods available to measure the drug-protein interaction?

Hello everyone, I would like to understand the interaction between my drug of interest to a protein (known). Basically, I want to study how strong the interaction is. Is there any way to measure...

10 November 2019 4,636 5 View

How to develop MCF7 mouse xenografts?

Hello everyone, I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA. My first question is, I wanted to develop the...

09 October 2019 4,774 4 View

Can someone please guide me or share the knowledge on how to generate sgRNA pool?

Hello everyone, I have an experience on CRISPR/Cas9-mediated genome editing. So far, I have designed more than 200 guides targeting different human genes and their efficiency have been validated...

06 July 2018 4,309 2 View

Could you please help me why my Flag-tagged gene is not expressing?

Hi all, Whenever I try to do immunoblot for FLAG or HA tag, I could not able to see their expression. Do I need to modify my protocol? I am successfully getting images for endogenous expression....

06 July 2018 9,846 10 View

How to get the information about the list of drugs under the clinical trial for a given disease?

Could you please help me to find the information about the list of drugs under the clinical trial for a given disease? I know my question is vague but any closest answer would be helpful for me....

04 May 2018 6,605 5 View

How to determine the intracellular drug concentration?

Hello all, I wanted to know the amount of drug present inside the cell after drug treatment. I checked articles for the same, finally I ended with targeted mass spectroscopy (MRM analysis). But in...

31 December 2017 2,455 3 View

No band for phosphorylated H2AX (gammaH2AX) when I treat with the drug?

Hi all, I have treated the drug that induces DSB to the cells (HEK293T). After 24 h of incubation, I harvested and did Western blot for gH2AX but I did not see any bands. I made 12% gel and...

11 December 2017 1,087 2 View

Is it necessary to synthesis second strand cDNA to amplify the gene of interest?

Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...

10 November 2017 9,318 11 View

Can someone please recommend me the protocol for the gamma H2AX foci formation assay?

09 October 2017 8,306 8 View

Problems in amplifying my gene of interest from the cDNA ?

Hello Everyone, I am trying to amplifying a gene from the cDNA. I used Superscript III to prepare cDNA and got 1.5 ug/uL concentration. For amplification, I used 50 ng of cDNA template. But I...

09 October 2017 3,712 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View