Can someone help me to calculate the masse of enzyme taken to obtain c=1000 u/ml

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Experiment or Quasi Experiment Design?

What kind of study design are we talking about? Subjects are randomly assigned to one of three groups. In addition, a moderator and a mediator are collected as continuous variables. Is this an...

15 January 2021 1,809 4 View

What is the best solvent for the extraction of chemical compounds that have an insecticidal effect ?

26 November 2020 4,609 15 View

Nvivo - how to extract and analyze hashtag?

Hello, I am using Nivo 12.0 plus. I've a dataset of tweets, unfortunately not extracted by NCapture. How can I extract and analyse hashtag? Is it possibile using auto-code functions? Thank you

19 August 2020 9,532 3 View

What is a scoping review?

In what context should we choose to do a scoping review? How is it different from systematic review, literature review, mapping review and Cochrane review? What are its mandatory or optional...

05 July 2020 8,206 3 View

If I use REVEL score as a meta-predictor for clinical variant classification, what would be a reasonable cut-off to filter disease variants?

Ioannidis et al., 2019, reported the development of the REVEL score (rare exome variant ensemble learner) for redefining pathogenic variant classification, and Tian et al., 2019, reported that...

29 March 2020 2,450 2 View

Is there a method to statistically compare two or more regression coefficients in paired data?

Hello, I have conducted simple linear regression for four time points, always on the same sample. My time points are 10 years apart and now I wish to compare the slopes / betas / regression...

21 March 2020 10,020 9 View

Is the Case Study Method eligible to create theory?

I have been conducting research for PhD Theses using case study as a method, mainly refer to Robert K. Yin works. At the final step of my research, I define some concepts and use the concepts to...

12 February 2020 3,146 10 View

What is the blue-shift in UV-vis?

hello, Im having trouble in extracting information from my uv-vis data. The sample the I analyzed is protein corona. Before that, I already characterize 40 nm of gold nanoparticle and obtain its...

15 May 2018 8,815 7 View

Does someone have neuronal cell cultures of zebrafish?

I plan to use immunohistochemical methods to stain brain sections from adult fish (teleosts, starting with zebrafish). In order to validate the antibodies I would like to test them in a 2D-celll...

10 September 2017 6,280 1 View

Please does anyone knows how to use genome mining in search of genes encoding for particular enzymes?

For instance if I want to search for genes encoding particular enzymes which bioinformatic tool is better and how do I select the best genes to investigate?

14 August 2017 1,967 4 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,311 5 View

What are the factors on which exchange current density of an electrode depends, and how can they be predicted?

While surface roughness is an obvious factor, this is not what is exactly asked. Rather, periodic variation of exchange current density (of an electrode) with solution chemistry (of electrolyte)...

30 January 2021 4,886 5 View

Is there any low-cost filtering system (or other tech.)to lower the salt conc. (salinity) of surface/groundwater for large scale agricultural system??

The available water (surface and groundwater) is highly saline (6-20 dS/m). It is required to produce crop (moderately saline tolerant, up to 6 dS/m). Is there any low-cost filtering system (or...

18 January 2021 5,015 3 View

Why does my Giemsa staining solution stay in the filter?

I wanted to use commercial Giemsa staining solution and diluted it 1:10 in pH 6.8 Weise buffer. It says to filter the solution, however, most of the stain seems to preticipate in the filter. The...

04 January 2021 5,028 2 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View

How to remove the bubbles in Collagen gel and maintain the transparency?

I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...

27 December 2020 5,495 3 View

How to determine the dominant orientation of collagen fibers.?

what is the best method to determine the dominant orientation of collagen fibers? i preformed FFT and shited the frequencies , what should be my next step ?

24 December 2020 3,360 3 View