Diane Leigh

2 Questions 6 Answers 0 Followers

Questions related from Diane Leigh

Has anyone seen a shift in the melt curve Tm after "siRNA knockdown"?

Cells were transduced with a shRNA lenti particle to knockdown a particular gene. Instead of seeing a knockdown in gene expression I got a shift in my Tm from 81.83 (in both CTL untransduced cells...

07 January 2017 6,344 3 View

Is there a trick to transfecting BV2 cells with siRNA?

I have tried several different reagents and monitored from 4 to 72 hours, I am not seeing toxicity, but I am not getting siRNA into the cell, no detectable GFP with contro and no knock down with...

17 July 2016 4,639 6 View