Hi everyone, can someone help me in understanding the antibody behavior under different conditions. Does antibody structure opens up, exposing its amine group for bonding under basic conditions (PBS buffer)?? Antibody in glycerol is closed confirmation?? and what about acidic environemnt. As, I want to conjugate digoxin antibody with carboxylic group of mercaptoacetic acid HSCH2COOH (MAA). I am using pure antibody followed by different dilutions in PBS buffer. Thanks.