My lab screens water samples for Legionella. This bacteria popped up and we have never seen anything like it. These colonies grew on BCYE agar plate containing glycine, cycloheximide, vancomycin, and polymyxin B. The sample was treated with a KOH/HCl acid wash pH 2 for 10 minutes. It also requires cysteine to survive.
While we have supplies to detect and confirm Legionella, we are unable to do any other microbiological tests on bacteria.
This publication might help in lowering the chances of getting other bacterial contamination
Article Evaluation of BMPA, MWY, GVPC and BCYE media for the isolati...
The bacterial contamination can be purified via repeated streaking forming a single colony and then the colony can be sent for identification to other companies that can do 16s rRNA sequencing followed by molecular phylogeny.
see
https://www.lcsciences.com/discovery/16s-rrna-gene-sequencing-for-identification-classification-and-quantitation-of-microbes/
Very difficult to tell from just the colony morphology. Are you definitely sure it requires cysteine? What do you use to confirm presumptive legionella, latex agluttination? Can you gram stain or use API?
Alicia Richarte .-. Did you find out any more about your contamination? Did the replies above help? Is it possible your contamination is fungal? Please let RG know if your post helped in any way.
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