Dear colleagues,
I am studying microglia in early development. I would like to design a study where I deplete microglia in neonatal pups using a CSF-1R inhibitor.
Several studies that employ the strategy of ablating microglia deliver these drugs via chow. However, neonatal pups cannot yet feed themselves, and there is no published evidence that sufficient traces of the drug can be passed via the maternal breast milk to deplete microglia in the pups.
Has anyone tried administering CSF-1R inhibitors via IP? If so, are there any specific protocols to prepare the drug for delivery? Does it need to be diluted or mixed in PBS or DMSO for example? How do you determine the appropriate dose to inject? How often should injections be administered?
I appreciate any helpful insights in advance.
Best,
Alicia
- To determine the appropriate dose to inject calculate the body mass of the neonatal pups and according to their mass value calculate the dosage and for your reference refer to this paper.
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- For inhibitors dosage analysis better you check the concentration of your inhibitor for diluting the inhibitors with the above-mentioned reagents.
IP seems to be the best option for administering CSFR1 inhibitors. However, I dont think you need to worried too much about the protocol and related stuffs just because your animal subject is neonatal pups. You just need to focus on two aspects:
1. Choose the best solvent for the inhibitors.
2. Choose the dose according to body weight. e.g., xyz mg/kg/day
I would advice you to do a pilot study on small number of animals to standardize these requirements.
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