Purchased kit to run on old iCycler but directions did not have a method. Only said to use CFX
Manager Software and select the appropriate thermo-protocol for the detection or quantification of Legionella. Looking for details about this protocol.
Just drop biorad an email. They are usually very responsive.
I am looking for a way to calculate the number of water molecules that are in a hydrogel sample.
16 May 2024 7,064 3 View
I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...
28 April 2024 3,860 1 View
Hello! I'm in the first stages of my thesis and, right now, I'm developing my research aim and objectives, as well as their respective research questions. I would like to study the significance of...
26 November 2022 5,760 3 View
Hi, I would like to measure the halo size of certain antimicrobial inhibition halos and was thinking about using ImageJ. However, I have tons of plates. Do you know of any plugin that can detect...
29 October 2022 4,800 3 View
Does anyone know the best way to analyse this set of questions using SPSS? For reference, my study is comparing the responses of university students and non-university students.
22 May 2022 719 3 View
I am looking anew into the area of what used to be called "scalable autonomy." I worked in this space briefly around 2014. Now, though, I haven't been able to find much associated with the terms...
07 February 2022 9,338 2 View
I prepare in-house media in Duran glass bottles, and am trying to find a way to seal them to prevent any tampering. I currently use shrink plastics post-sterilisation (either autoclaving or filter...
09 June 2021 8,105 1 View
I’m currently trying to develop a 10 SSR marker multiplex for allelic screening using the universal tags and the method (iii) from Blacket et al. (2012 - DOI: 10.1111/j.1755-0998.2011.03104.x )....
14 March 2021 3,069 1 View
Dear colleagues, I am studying microglia in early development. I would like to design a study where I deplete microglia in neonatal pups using a CSF-1R inhibitor. Several studies that employ the...
06 November 2020 997 2 View
Hello, I'm a first-year graduate student, and my lab is looking to get into whole-mount immunostaining of cleared mouse tissues (specifically, at this point, lung and skin). I'm very new to this,...
22 September 2020 8,786 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View