What is PCR amplification protocol for iQ-Check Quanti L. pneumophila Kit?

14 November 2020 1 9K Report

Purchased kit to run on old iCycler but directions did not have a method. Only said to use CFX

Manager Software and select the appropriate thermo-protocol for the detection or quantification of Legionella. Looking for details about this protocol.

Badges
Science topic

More Alicia Richarte's questions See All

Does anyone have experience giving a CSF-1 R inhibitor via IP injection to deplete microglia?

Dear colleagues, I am studying microglia in early development. I would like to design a study where I deplete microglia in neonatal pups using a CSF-1R inhibitor. Several studies that employ the...

06 November 2020 793 2 View

Best fluorescent antibodies for whole-mount staining of mouse tissues?

Hello, I'm a first-year graduate student, and my lab is looking to get into whole-mount immunostaining of cleared mouse tissues (specifically, at this point, lung and skin). I'm very new to this,...

22 September 2020 8,582 3 View

Which are the normal levels of body fat in children estimated by anthropometry?

I am looking for knowing the levels of body fat (BF%) estimated by 4 skinfolds (biceps+tricipital+subscapular+ suprailiac) in children aged 6-12y . Thanks!

15 January 2020 8,376 7 View

Can anyone suggest some links where i can get free access to Synthetic Aperture Radar Images for research purposes?

20 December 2019 6,961 4 View

Can someone suggest or refer an effective procedure for the removal of a sample from Wax, so that it can be embedded in EPON for SEM?

We require a method that will keep the sample in tact while removing it from Wax embedding, so that it can then be embedded in EPON in order to avoid damage to the instrument during scanning...

19 November 2019 9,851 2 View

How do you best store isolated exosomes?

I am isolating exosomes from the SBI Exoquick TC Ultra kit. I'm going to run some right away on western blots, but was hoping to store some for a while to add onto gelatin matrices for imaging....

03 October 2019 6,254 3 View

What is the procedure required to get access to the publicly available MSTAR dataset?

31 March 2019 726 1 View

Before anything else..Thank you! I actually want to do a research on positive psychology.. Any interesting topic as your suggestion?

I want to do a research on positive or even in existential psychology that may add to my learning the field of psychology.. I just want to source out topics from experts like you. Thank you.

19 January 2019 9,698 6 View

What is as effective method to teach quick and accurate ELISA plate loading?

My new tech is so slow at adding saliva samples to competitive ELISA plates. I thought after a week of loading two full 96-well plates a day he's be more comfortable but he's using two hands to...

16 June 2018 2,404 3 View

Any recommend journals for social media influence voting behavior and perception?

Hi there, I'm currently working on my thesis on the topic of "How social media influence voting behavior and perception of youth". Hope to get some recommendation of journals to work out on this...

16 December 2017 4,030 4 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View