I have been using PCR for a quite long time of HBV, HCV and HIV, we always used to get the extraction protocol and kits with every individual virus..reagents are same though...this time during pandemic..there is some shortage of extraction kits for covid 19, since SARs - CoV 2 and HCV both are RNA viruses I am planning to use HCV RNA extraction protocol..would anyone share their experience or guide me?
I would recommend trizol for RNA extraction. inexpensive and easy.
TRIzol Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.
Here I include this guideline....................... Please take a look.
https://www.westburg.eu/theme/coronavirus-covid-19/viral-rna-extraction
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