Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

I am proposing to write on effect of stigmatisation and mental illness among BME in Uk. How can I rephrase this pls? Any material on this pls?

I am proposing to write on Stigmatisation and mental illness among the Black and Ethnic Minorities living in the Uk , mostly the Afro-Carribeans. I would like to know how I can re-phrame the...

17 August 2020 3,809 8 View

Species identification from images: Flying fish, cephapods and jellyfish?

Hello there, We have a number of images taken at the surface and during blackwater dives during an expedition to the Sargasso Sea, August 2019. I am seeking experts that might be able to identify...

06 July 2020 4,958 4 View

My logcombiner doesn't work, either version within BEAST 2.5 or 2.6! Any ideas for an online platform, or perhaps a package in R?

I am trying to combine two runs from BEAST 2.6 and strangely my Logcombiner doesn't open. Surely there must be another package that can perform the same function? I have looked for earlier...

19 March 2020 7,701 3 View

What is the rationale of heart rate and temperature monitoring with respect to internet of things?

The relationship that exist between the heart rate ,temperature and internet of things

07 November 2019 7,690 3 View

What caused a western blot to show total protein even after stripping and blocking?

I just finished up a western blot with two specific antibodies (one of which was for tubulin) where, when reading on a 700/800 fluorescent Odyssey scanner, my results look the same as my Coomassie...

03 May 2019 6,286 3 View

How can I further reduce formation of dimerisation/oligomerisation of GPCRs during my Western Blot?

Hello all, I am attempting to compare degradation of a GPCR, specifically the Dopamine D2 receptor, with a mutant form of the receptor I have created, with extended agonist treatment. Both...

24 March 2019 1,058 3 View

How do I respond to full-text requests without posting publicly for everyone?

I received a full-text request through RG and clicked the link from the RG email and it brought me to a prompt where I was forced to either agree to publicly post the paper for everyone to see, or...

21 March 2019 3,017 3 View

How do I unscubscribe?

This is not what I thought Research Gate was. I want to unsubscribe since I have no time for social media. Cheryl

14 February 2019 9,401 1 View

What is a good neuronal apoptotic precursor?

I am trying to find a way to look at damage done after hypoxic stress. I have looked into HIF 1 alpha and the Caspases but I feel like there is a better ones out there that has an early onset and...

13 December 2018 9,872 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View