I have precipitated saponins from methanol extract with acetone. Now, how do I purify these now
Use resin like Diaion HP-20, XADs followed by Sephedex LH-20, C-18.
If you havnt C-18 then you have go for Silica (60-120) 0r silica (200-300)
gudluck
:)
if your saponins is not pure, then you can go again for acidification of product in methano up to pH 6-7, filter it again and immediate wash with ether, acetone nad methanol, by this you can save yield loss by purification.
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