Can someone advise on how to get bands with chloroplastic genes for phylogenetic analysis of euphorbia species?

Saba Saboori @Saba-Saboori

15 January 2014 1 930 Report

the PCR program is as follow: 94 C 5min, 35 cycles [(94 C 50 ") (58 C 50") (72 C 1min)] (72 C 7min)

(trnH forward and psbA reverse primers)

Nadine A. Gund

Hi, first of all:

How did you extract your DNA? Is the quality and quantity okay? Did you check it?

How many DNA did you use for PCR?

Are all your PCR-reagents okay?

Did the PCR-program ever show any bands? Did you design the primer?

...

Cheers, Nadine

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