I want to do structural characterization of my recombinant protein which is going into inclusion body
Interesting that you are considering the inclusion body to have a particular structure. It will be a mess of amino acids. Anyway I would freeze-dry the material for your analysis.
currently working on a project involving Daphnia magna and I am intertied in measuring their total lipid concertation. I was hoping to get some insights or advice on the standard procurers for...
23 May 2024 8,846 1 View
what is 1335 in acetyl value and why this value put here as constant? what is 1335 in acetyl value and why this value put here as constant?
10 January 2024 8,357 0 View
Hello, Researchgate community, I have one query regarding deciding whether our electrode material is suitable for practical energy storage applications. When we prepare and characterize...
22 October 2023 6,027 3 View
I'm trying to induce oxidative stress by using rotenone and SNP sodium nitroprusside in isolated mitochondria of rat brain. This is to study GSH assay from control (Mitochondria) group in...
11 June 2023 6,247 2 View
While doing GSH on rat mitochondria, I have calculated protein concentration by Bradford's assay (1.35mg in 2ml buffer). In case of GSH assay I got concentration of 0.23mM (GSH) in mitochondria....
03 May 2023 2,820 6 View
I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation. Therefore,by In-vitro model how can I take the volume...
07 February 2023 2,865 0 View
Rotenone is the insecticide used as ROS Inducer in mitochondria. In aiming that what will be the method for the preparation of calibration curve for rotenone?
07 February 2023 5,471 0 View
07 February 2023 6,362 4 View
I have a thioredoxin tag with my therapeutic protein. I have purified it using Ni-NTA. Next, I want to check it's activity by Elisa or cell proliferation assay. Is it necessary to remove the tag...
11 November 2021 6,284 10 View
I want to biotinylate polyclonal antibodies. The application of these polyclonal antibodies would be in the lateral flow assay. I am not looking for any commercial kit. Please help with the...
23 September 2021 8,241 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...
09 August 2024 7,892 0 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View