Hi Charalambos,

If i recall correctly, 3D iterative tresholding is one of the functions included in the 3d ImageJ suite plugin. This plugin has been published (see link). The paper itself is not very detailed but the supplementary info is quite extensive and might contain some info on the iterative tresholding bit.

Hope this helps

Cheers,

Pieter

Hi Pietre,

I have already read all the aforementioned infos and are not as technical, as I wanted them to be. Since I might use this segmentation method for scientific publication, I would rather to have more details to explain the method.

Regards,

Charalambos

http://cn.mathworks.com/matlabcentral/fileexchange/56371-zhenzhou-threshold-selection

I hope that this threshold method link might help you.

Hi Zhenzhou,

Very interesting method the one you 've developed. My question though is: Which exact iterative thresholding (3D) method does the ImageJ (FIJI) use?

Does it use the otsu, EM, kmeans (this one is actually used when is chosen)?

More specifically, when you choose a "Threshold Method" the "Step" method. What does it actual mean with that? Could it be isodata? :/

C