We have Two types of DU145 cells in our lab and I am badly confused which one to use. So, Please upload some photos of DU145 so that I can come out of the confusion.
Thank you
http://www.medsci.org/v08p0097.htm
Try this link:
http://www.lgcstandards-atcc.org/~/media/Attachments/2/B/A/9/HTB-81%20High%20Low.ashx
From: http://www.lgcstandards-atcc.org/products/all/HTB-81.aspx?geo_country=es#generalinformation
Hope it helps
I have the image of that cell line under the culture condition of RPMI1640 with 10% FBS, would that be useful?
yes . plz send
Hello everyone, In eukaryotic cells, all the mRNAS are polyadenylated at 3' end. I want to ask whether this is the same case for pox viruses also. Do all their mRNAs are polyadenylated at 3'end...
04 May 2019 3,309 2 View
Hello everyone. I want to do DNMT activity analysis by using prostate cancer cells. I want to know whether we can store the nuclear lysate for 2-3 days in -20 degree before performing activity...
05 June 2018 6,568 7 View
Hello everyone I have bisulphite converted my DNA using kit from Zymo research now I have to do methylation specific PCR. I have to check effect of my drug on methylation status of diff. genes...
10 November 2017 8,380 4 View
I am going to do ms pcr but I have doubt on water milliQ water because previously RT pcr result was not coming by using that water. So I borrowed water from another department but that water was...
09 October 2017 4,011 4 View
Hello everyone, I isolated DNA from freshly taken Human blood but during processing I was getting RBC contamination in pellet .I washed with RBC lysis buffer 6-7 times followed by...
09 October 2017 5,451 3 View
Hello, I have 3- 4 month old DNA stored at - 2O degree. Can I use thisDna for bisulphite treatment followed by methylation specific pcr? Thank you
31 December 2016 5,366 5 View
Hello, I want to do radioactivity based DNMT activity assay. Can somebody upload the whole prtocol for this? Thank you
06 July 2016 2,258 5 View
I have done RT -PCR for Cyclin D2, p21 and p27 in du145 , but did not get any band . in p21 , there was v. faint band but when I repeated the same , I did not get any band for p27. does these 3...
03 April 2016 928 1 View
What is the best way to freeze LNCaP cells? ATCC recommends complete medium plus 5% DMSO. some people use 10% DMSO, 20% FBS and rest complete medium. Some use 90% FBS and 10% DMSO. Please tell me...
31 December 2015 2,459 3 View
we purchased RCCS from synthecon Inc. In users guide it is mentioned to prepare vessels by rinsing with media or PBS for 5 mins to overnight. I want to know the purpose of doing this...
31 December 2015 4,483 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/
08 August 2024 4,585 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View