First Issue is : Patient viral load for Influenza flu virus (according to clinical case studies). What is the initial viral load patients come to doctor with (especially for diagnosis in the current times for H1N1 or H3N2 cases).
Second issue is : Most used RT-PCR primers for detecting the viral particle number in Influenza H1N1 and H3N2 cases.
Third is : Reference paper to convert HA units of Influenza virus to viral particle number. How are both correlated?
This articles will help you
Influenza vaccines for the future.
Lambert LC, Fauci AS.
N Engl J Med. 2010 Nov 18;363(21):2036-44.
The evolution of seasonal influenza viruses.
Petrova VN, Russell CA.
Nat Rev Microbiol. 2018 Jan;16(1):47-60.
New insights into influenza A specificity: an evolution of paradigms.
Ji Y, White YJ, Hadden JA, Grant OC, Woods RJ.
Curr Opin Struct Biol. 2017 Jun;44:219-231.
Re your third query, this is the relevant section from the notes of a practical class helped teach to undergrads in Cambridge
The infectivity assays for animal viruses are less efficient and we find for fowl plague virus, for example, that very approximately : 1 HA unit = 107 particles = 106 EID50 = 105 p.f.u.
(Fowl plague = avian influenza)
1 pfu = 10-100 particles.
the numbers could vary by ten fold either way perhaps, depending on how you set your HA assays up, how well the virus stock was prepared and the exact strain of flu, but it’s a ballpark figure.
paul
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