Chandan Narayan

5 Questions 19 Answers 0 Followers

Questions related from Chandan Narayan

Can Somebody let me know some best reference papers for three of the issues?

First Issue is : Patient viral load for Influenza flu virus (according to clinical case studies). What is the initial viral load patients come to doctor with (especially for diagnosis in the...

16 March 2018 7,281 4 View

Is Bmax value greater than 1 correct in a radioligand binding assay?

I use sigma plot software, its a radioligand binding assay for determination of Kd values for the Aptamer-protein binding. I use one site saturation.

06 March 2018 5,506 3 View

How to remove the unincorporated labeled nucleotides from the sample eDNA ?

I have been using illustra microspin G50 columns for getting rid of the unincorporated labeled nucleotides from my sample eDNA. But unfortunately this does not seem to work. I still end up with...

09 February 2017 4,783 9 View

Filter Binding assays help needed

I am into SELEX to get aptamers for my target. I have been doing eDNA-protein interaction studies with gamma-p32 labelled isotopes to my eDNA. When i try to estimate the amount of labelled eDNA as...

09 November 2016 5,233 12 View

Can furin be expressed effectively in insect (SF9) cell line? Trying to make a continuos cell line expressing furin. Suggestions needed?

Furin is expressed as a proprotein, so i had included a myc tag after its cleavage site on the n terminal position. have confirmed the tag by sequencing. Aptly, i need to see the band on a western...

01 January 1970 10,043 2 View