Chandan Narayan

3 Questions 8 Answers 0 Followers

Questions related from Chandan Narayan

Is Bmax value greater than 1 correct in a radioligand binding assay?

I use sigma plot software, its a radioligand binding assay for determination of Kd values for the Aptamer-protein binding. I use one site saturation.

06 March 2018 5,292 3 View

How to remove the unincorporated labeled nucleotides from the sample eDNA ?

I have been using illustra microspin G50 columns for getting rid of the unincorporated labeled nucleotides from my sample eDNA. But unfortunately this does not seem to work. I still end up with...

09 February 2017 4,566 9 View

Can furin be expressed effectively in insect (SF9) cell line? Trying to make a continuos cell line expressing furin. Suggestions needed?

Furin is expressed as a proprotein, so i had included a myc tag after its cleavage site on the n terminal position. have confirmed the tag by sequencing. Aptly, i need to see the band on a western...

01 January 1970 9,851 2 View