Can somebody give me a protocol to prepare hatching enzyme solution?

02 February 2015 1 8K Report

I want to dechorion sea bass eggs to stablish a embrionic cell line. I have thought that the best thing is to obtain embryonic cells eliminating the chorion. Maybe another alternative is possible

Is somebody able to help me?

Dr.S.N. Sethi

Dear Dr.Carlos,

You can try some other catfishes and salmons for isolation of Chorionase enzymes, i think mouse or other lower vertebrates may have this enzyme for establishing embrionic cell lines. We have seabass eggs in our hatchery, you can tell us at what stages of embryonic cells can be taken ...

Regards

Dr.Sethi

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