Can somebody get this paper?

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Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

Dataset of synchronized cardiac angiography and ECG?

Hello, I'm working on medical project and I would need synchronized angiography with ECG? Does anyone know if some open source dataset of this kind exist? Regards, Bruno

25 July 2024 2,214 2 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,073 2 View

How to adjust the survey weights for non-response rate in a stratified random sample design?

Thank you in advance for your support. We conducted a stratified random survey in 13 strata and obtained a sample size for each stratum. At the time of the survey we had a variable...

01 July 2024 281 3 View

How to reduce the material coming out of the torque rheometer?

A physical mix was in vitro evaluated on a torque rheometer. The composition was predominantly recycled polypropylene (PP), yet the melt flow index (MFI) was found to be excessively high....

25 June 2024 10,154 0 View

What are the best ways to the recount of microalgae?

I know the neubauer method, but I hear about the recount of growth with spectrophotometer.

23 June 2024 9,295 1 View

Cheaper alternative to XSens mocap system for ergonomic data acquisition?

Hello all, I'm starting a project where we want to automate the video analysis of people working in different environments to produce ergonomic measures like hip flexion, shoulder extension, etc....

16 June 2024 1,843 1 View

Does anyone have experience with immunofluorescence of endoplasmatic reticulum?

Hi! I have performed several immunofluorescences of cultured ARPE-19 cells with different antibodies expecting in each one a cytoplasmic localization. However, it seems to localize in the...

06 June 2024 7,764 1 View

How can I change the coordinate system in Abaqus using Python?

Hello, I'm writing Python scripts for Abaqus and I'm facing a problem. I need to change the coordinate system in a .odb file before extracting data but I'm stuck. I can create my coordinate system...

30 May 2024 7,431 3 View

How can I induce neuroinflammation with LPS in HMC3 cells ?

dear all, I try to induce neuroinflammation in HMC3 cells using LPS for 24h. The concentration used was 500 ng/ml. But unfortunately no change on TNFa was obtained in LPS versus Control...

29 May 2024 4,007 1 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Can type 2 diabetes be reversed at any age through proper diet and exercise?

Type 2 diabetes can be managed and, in some cases, put into remission through proper diet and exercise, regardless of age. Here are some key points: Weight Management: Achieving and maintaining a...

07 July 2024 8,745 2 View

Issue with micrometer beam size while simulating moving heat source problem?

I am trying to replicate results published in the journal paper published recently (Modeling of Laser-assisted micro-milling Inconel718 ). In that paper, they used vdflux subroutine to obtain...

05 July 2024 3,701 0 View

How do I control powder volume in a mechanochemical reaction?

Here is one case: I want to perform X + Y in the presence of Z catalyst in a 50 mL milling jar with 8 12 mm milling balls and investigate whether the the amount of catalyst affect the yield of the...

18 June 2024 2,706 1 View

Why does the color of silica gel changes significantly when milled in stainless steel jar?

Whenever I mill silica gel with other materials in stainless steel milling jar, the color of the powder goes from white (initial) to dark and darker color like yellow, dark yellow, brown, dark...

16 June 2024 3,984 3 View

Is there any technique which can help in determining sugar concentration in water?

"I want to determine the concentration of a series of sugar solutions. I tried to use refractometry, but the concentration is very low and the difference between the refractive index (RI) values...

29 May 2024 2,464 9 View

Why don't powder cell culture medias include sodium bicarbonate?

I'd like a powdered form of MCDB 131 Medium with sodium bicarbonate added, and I've noticed that not only is the powder form of this medium difficult to find with NaHCO3 already included, but the...

28 May 2024 1,631 2 View

Is there an HSQC sequnce suitable for 13C labelled small molecules?

I work in glycobiology and one of my duties is synthesize short oligosaccharide with, at least, one sugar universally 13C-labelled. Is there any sequence that can help me with characterization,...

28 May 2024 8,039 0 View

Waste products of various fruits and vegetable are generally thrown away but these can be used as useful chemicals like Ethanol by sugar ind efflu?

Various Waste materials as biomass of Plants and Animal,have different organic Complex compounds as Proteins,Carbohydrates,Fats,Lipids,Vitamins and more Minerals .As Carbon di oxide ,water and...

27 May 2024 3,186 1 View