When drawing a phylogenetic tree with somatic mutations(SNPs), what is the role of tree simulation process?

Hello, I am working on drawing a phylogenetic tree with samples sharing some somatic mutations. The basic process of my work is similar to this article. Population dynamics of normal human blood...

14 October 2020 8,078 4 View

In grignard reaction, how can I attach 'Mg' to only one side of dibromoalkane?

As you can see in the image file, I want to make Br-C12-dithiocarboxylic acid form. However, I don't know how to control the amounts of reactant. I tried 'Mg:alkane=1:1 equiv' but many byproducts...

18 September 2020 3,970 4 View

I would like to mix FBS and horse serum (1:1) when culturing cells, do you think it would change sequencing results?

Hello, I am doing fibroblast, stem cell culture for sequencing. (From primary culture to the end) Since FBS is expensive, there are some protocols that mix FBS and horse serum (1:1) I have done...

10 September 2020 8,137 3 View

Is there any way I can do spheroid culture with fibroblasts (not cancer cell)?

I have done spheroid culture with various cancer cell lines The spheroid was formed from single cells. The method was like the added picture (not curved bottom well, matrigel, etc) The question...

30 March 2020 9,817 3 View

Why use dol dme electrolyte in lithium sulfur batteries mostly?

In lithium metal battery with transition metal cathode, they use lipf6 salt and different electrolyte. However, in lithium sulfur batteries, almost studies were conducted under dol dme electrolyte...

30 March 2020 9,603 4 View

Some of SH-SY5Y cells detach during media exchange in 96well How can I deal with?

Hi all, I seeded 1 X 10^4 of SH-SY5Y on 96 well plate and grew them overnight. For drug experiment, I removed all media using pipette and treated media mixed with drug. However, I found that some...

23 March 2020 8,924 4 View

Would you let me know how to extract Polysaccharides?

I'm planning to extract Polysaccharide from Codium fragile, which is green algae. I used to use hot water for extraction for 3 hours and then use Ethanol to get them. I tried this way, but It...

17 September 2019 4,558 5 View

About photonic crystal, Is there any conditions that minimum number of polymer layers of particle which can color?

I understand that incresing number of layers causes high reflectance..but I don't know the minimum condition that particle can be used as photonic crystal..

08 August 2019 1,413 1 View

Vimentin Western Blot: No consistency of the change in vimentin expression level of A549 cell line. What can I do?

I am working on Epithelial to Mesenchymal Transition (EMT) in A549 cell line by treating positive inducers such as EGF to up-regulate the mesenchymal markers like vimentin. I'm doing western blot...

22 May 2019 3,053 1 View

Can I use this live/dead cell assay kit after I fixed the samples?

https://www.thermofisher.com/order/catalog/product/L3224 This is the live/dead cell assay kit I will use for my experiment. But I have to fix samples (cancer cells) before using this kit, maybe...

29 April 2019 3,481 3 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Simulation or Experiment? How do we choose?

If we want to assess if a phenomenon is going in a direction we expect, we can simulate it, using digital models, possibly agent models factoring in known properties and interaction features. We...

12 February 2021 1,141 21 View

Vth increase with programming SONOS Flash in Tcad?

Hello, I've been working on this issue for more than a month now.... I made a basic SONOS flash structure using TCAD Sentaurus sProcess. (tunnel oxide/nitride/blocking oxide : 4/6/6 [nm]) Then,...

11 February 2021 3,337 3 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View