How mirna chose its target among 100s of mRNA having seed sequence?

I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...

03 April 2019 7,825 5 View

How to meassure primary miRNA trancript using QRT PCR?

I want to know how to design primer for primary miRNA QRT PCR

02 March 2018 220 7 View

Can I store phenol chloroform phase in trizol?

I use trizol for RNA isolation,but we store phenol choroform phase -30 , I want to know is it good to do isolation of protein later after storage -30 of the above mentioned phase or it may affect...

10 November 2017 7,410 0 View

Anyone used mirna mimics cholesterol conjugated for primary B cells?

I read recently that cholesterol conjugation remvoes need of lipofectamine and enhance efficiency of transfection of mimics.If any one has used please answer

11 December 2016 6,773 1 View

Can miRNA mimics or inhibitor used in primary cells?

I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells

10 November 2016 3,344 4 View

Can i use different concentration of forward and reverse primer in real time ?

i am currently making assay of syber green based real time pcr for detecting rare isoforms.To make them specific ,can i use say 1 p mole of forward and 5 p mole of reverse primer,technically for...

06 July 2016 8,260 4 View

Can anyone provide a table for miRNA and respective gene from which it arises?

There are certain miRNA originated from the intron of genes. I could not found such tabulated form in which genes and respective miRNA are given Can anyone provide the same.

11 December 2015 1,564 3 View

In rel time PCR non specific band at higher dilution?

09 October 2015 6,164 3 View

What is meaning of miR17 approximately 92 cluster?

During literature search I found miR 17/92,miR-17 approximately 92, miR 17~92 I am not able to understand practical meaning of these annotations in microRNA. Can anybody help to me to understand...

09 October 2015 6,743 1 View

Can anyone help me with the interpretation of intron centric analysis file output ?

RNA seq data analysis by intron centric analysis gives insight about exon skkiping ,I have attached photo of excel file I have. Can anyone help me how to interpretate it eg...

06 July 2015 9,479 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View