Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What is the full script that I can use to extract sequences from fasa file by using sequenc IDs in text file using python 3 in pycharm environment ?

I want to extract sequences from fasta file using a text file witj ids in python

29 May 2020 1,674 3 View

Measure the Real area when tilt SEM stage?

03 April 2020 7,068 3 View

Extraction of peptides from hemolymph of insect?

I am really confused about this step, every single paper i read have a different methodology. Now i have samples of immunized hemolymph and i want to extract peptides from these samples. first i...

01 December 2019 3,621 2 View

What is the Future of bioethanol as a road fuel?

What is the future of bio-ethanol as a road (car) fuel? Do you think electric vehicles will take over the market very soon that no need for biofuels for road transportation?

17 May 2019 7,000 6 View

Can the same arrangement of small vectors of three level NPC invterter used for both high and low speed operations of DTC of IM?

Hi all, can anyone provides me with an explanation of utilizing the small vectors of 3L-NPC inverter for both high speed and low speed operations of Direct Torque Control of Induction Motor? In...

26 March 2019 8,994 1 View

How to submit your research manuscript to more than one publisher at the same time?

I found an interesting web site that allows the researchers to legally submit their manuscript to more than one publisher at the same time. The publishers will have initial screening and come...

19 December 2018 3,906 13 View

Can I detect amino acids by biuret reagent during separation on agarose gel electrophoresis?

During separation of amino acids using gel electrophoresis, can I use Biuret reagent or Ninhydrin.

14 November 2017 6,552 4 View

Starting a new publishing establishment for open access journals

31 January 2017 232 23 View

Health financing strategies to universal health ?

Does any one have studies or articles about developing health financing strategies to assist the implementation of universal health coverage Thanks

03 April 2016 7,515 9 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View