I want to construct an shRNA-plasmid containing multi-domains of a single gene with one hairpin and under the control of single promoter. Is it possible?
No
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I am interested in publishing an updated version of my final master´s project in Spain and i am not sure which steps to take. I am open to suggestions.
16 July 2024 7,431 1 View
Hi everyone, There are challenges with using antibodies to capture my protein of interest so I will be using CRISPR to endogenously tag my RNA binding protein with a fluorophore followed by a His...
28 March 2023 8,638 1 View
I transformed 5-alpha E.coli and plated onto freshly made agar plates with ampicillin (which has recently been made by another member of the lab). Less than 24 hours later I checked and my plate...
27 March 2023 4,425 6 View
How can I determine appropriate current density value theoretically for cathodic electrodeposition process?
14 August 2022 6,395 1 View
Dear All, I have an issue regarding a sediment transportation simulation. I have computed the sediment transport rate based on a 20 years wave climate, and simulated the sediment drift for the...
10 July 2022 8,926 2 View
Hi everyone, I have a question and I was hoping to get some insight from you. I ran RNA-seq on my samples and I didn't have replicates, I used differentially expressed genes with a cut-off of...
01 June 2022 1,350 2 View
I have a system consisting of 4 bolts that apply a compressive load on an item between two plates. I want to calculate how much the compressive load of the bolts decreases because of thermal...
04 September 2021 3,065 1 View
Hi everyone, I am planning to use TGF-β to transform my fibroblasts into CAFs. Most of the papers I have been reading have used TGF-β from R&D Systems, but none of them has mentioned whether they...
10 May 2021 733 3 View
Hi Dose any-one knows about cleaning validation protocols for pathogen-bacteria from fermentor? In industerial size, 1000 lit?
15 December 2020 4,685 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View