I want to construct an shRNA-plasmid containing multi-domains of a single gene with one hairpin and under the control of single promoter. Is it possible?
No
Hi Dose any-one knows about cleaning validation protocols for pathogen-bacteria from fermentor? In industerial size, 1000 lit?
15 December 2020 4,418 3 View
Hi what's the best DNA electroporation program for immature dendritic cell ?
17 September 2020 5,379 3 View
Dear All scientists; How can we calculate the concentration of CO2 emitted in the air in PPM? shall we use PPMv(Volume PPM) or Mass PPM is also acceptable? Regards
01 August 2019 7,464 4 View
When I save as a modified style and change its name, the new style does not show up with the name I have entered for in the styles list. In stead, a general title like "APA-Saeideh" is built...
20 May 2019 6,622 6 View
I have one factor as time (3 time points) and the other as treatment( absorbable versus nonabsorbable Sutures that each of them were used on different sides of a scar) so two treatments had been...
08 June 2018 6,220 1 View
How can I make a teeth whitener in the chemistry lab?
02 June 2018 6,306 3 View
Recently we performed expression and extraction of Nef proteins from Bl21 strain (His tag-Nef and Nef-GST), when we run these proteins on sds-page, we observed that His-Nef stay higher than...
21 January 2018 9,629 4 View
I am trying to make a ketogenic diet using lard and wonder how to dissolve lard and have a solidify mix of carbohydrate+protein+ lard at the end for 1 litre?
29 May 2017 7,074 3 View
Why high symmetry solvents are proper for freez polymer blends?
29 December 2015 3,684 3 View
I have two chambers, the inner contain a capillary tube and the outer chamber contains water to maintain the humidity. My problem is that when I fill the capillary tube (by capillary action) and I...
16 September 2015 1,293 7 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View