Saeideh Milani

3 Questions 2 Answers 0 Followers

Questions related from Saeideh Milani

Can shRNA-plasmids contain multiple domains of a single gene with one hairpin and under the control of a single promoter?

I want to construct an shRNA-plasmid containing multi-domains of a single gene with one hairpin and under the control of single promoter. Is it possible?

06 March 2014 2,562 1 View

Why I can not find my insert (80 bp shRNA) in plasmid (5000 bp pRNAi) via colony pcr?

I do not detect my insert in my colonies via colony pcr. What is the reason? I wanted to clone a shRNA in pRNAi. I digested my plasmid & pcr product of shRNA with the same RE. Then ligate them...

21 September 2013 3,717 1 View

How long does it take for tetracyclin to induce the RNAi vector and knock down the gene?

I transfected a cell line with pRNAi vector and then added tetracyclin as an inducer of its promoter. How long do I have to wait if I want tetracyclin to induce the promoter of my plasmid and...

21 February 2013 7,349 3 View