Ajwain (Trachyspermum ammi L.) seeds are small. I want to create pellets of 5-10 mm ? How to pelletize for increasing size? Suitable materials?

Ajwain seeds are very small. The seed rate per ha is 2.5 kg for row sowing with seed hopper. The seed should also needs shallow sowing. Usually fine sand or rice semolina mixed with seed and sown....

04 September 2020 4,706 1 View

What is the viability and time taken for turmeric seeds?

Turmeric is a polyploid. Mostly sterile. However, it sets seed occassionally. I have collected seed and kept in poretrays filled with cocopeat. I have not observed any germination even after 15...

28 August 2020 8,342 4 View

Can curcumin flourescence be used for detection and quantification of curcumin in live and extracted samples?

LMSC imaging used in many samples for flurescence of live animal samples. Can we use this technique for detection and distribution of curcumin in live plant tissues?

19 August 2018 4,316 4 View

Does anyone have any information on concentration ratios for monoclonal antibody against antigen for antigen capture?

Is there a standard ratio of concentration of monoclonal antibodies against a particular antigen for an antigen capture assay? To put it plainly how much monoclonal antibody is needed to bind to...

10 September 2014 7,365 8 View

Where can we get current hyperspectral remote sensing images?

We are planning to develop spectral signature library using hyper spectral remote sensing.

06 February 2014 5,658 6 View

Why is the sample moving in both sides in agarose gel electrophoresis?

The samples where - DNA isolation from liver, bacteria, cauliflower. The bacterial DNA sample are moving in the opposite direction to the liver and cauliflower samples.

19 December 2013 8,989 26 View

How do we estimate actual evapotranspiration from the given remote sensing image?

We are working on rainfall runoff modelling for Krishna river basin. We are interested to do the estimation of actual evapotranspiration from the remote sensing image using software.

13 September 2012 334 31 View

In M1V4 generation, selected a few mutatnts performing better than parents for yield? How to proceed to develop variety from this stage?

In 2016 I have developed a set of mutants in two varieties of turmeric-Mydukur and Prathibha. Now, in M1V4 evaluation, have identified a few are performing better than the parents. I am going to...

01 January 1970 4,130 4 View

Do turmeric rhizomes have dormancy?

I have observed, in India, in coastal humid tropics turmeric show sprouting around 2nd week of May which continues for another 45 days. Turmeric is usually harvested in this part during them...

01 January 1970 5,202 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View