How to prepare Peroxtesmo KO solution?

26 February 2022 0 6K Report

I wish to prepare peroxtesmo KO solution for studying stigma activity in plants. Kindly suggest how to prepare the solution.

Badges
Science topic

Similar topics
Geoscience
Asia
India

More Giridhar Kalidasu's questions See All

What is the best approach to establish entomopathogenic fungi in a crop ecosystem?

There are many entomopathogenic fungi available like Metarhizium, Beauveria , Verticillium which are used for control of several insects. It is apparent that these fungi do not establish well in...

26 November 2021 5,799 10 View

Is it necessary to use k-fold in BPNN-BR network with around 2k data?

I am trying to train a Back propagation NN with Bayesian regularisation with input vector has 10 features and single output of continuous value between(-1 to +1)(regression). It is a shallow...

13 October 2021 6,985 3 View

Mutation breeding (EMS) with single bud chips in turmeric, deleterious sprouts died, but given care, new sprout survived. Utility of this method?

In mutation breeding with EMS, turmeric single bud chips were used. Near LD50, many sprouts started withering. But due to care in handling like helping the pseudostem to open, helping the leaves...

16 August 2021 8,987 4 View

What is the relation between seed weight or size with the early seedling biomass and growth?

I want to screen fenugreek germplasm for early seedling growth, particularly biomass. If biomass of seedling, during early stages like 7-21 days, is associated with seed weight or size, then I...

01 July 2021 221 7 View

Ajwain (Trachyspermum ammi L.) seeds are small. I want to create pellets of 5-10 mm ? How to pelletize for increasing size? Suitable materials?

Ajwain seeds are very small. The seed rate per ha is 2.5 kg for row sowing with seed hopper. The seed should also needs shallow sowing. Usually fine sand or rice semolina mixed with seed and sown....

04 September 2020 4,895 1 View

What is the viability and time taken for turmeric seeds?

Turmeric is a polyploid. Mostly sterile. However, it sets seed occassionally. I have collected seed and kept in poretrays filled with cocopeat. I have not observed any germination even after 15...

28 August 2020 8,541 4 View

What are denaturing agents naturally available in plants? Particularly from edible plants?

Covid has protein capsid. Denaturing it would easily expose the RNA which denatures easily. Now, I am thinking to use some denaturating agent of plant origin, particularly from edible plants for...

05 August 2020 8,099 5 View

Can Raman spectroscopy be used in COVID19 qualitative testing?

I have read that non-destructive raman spectroscopy is useful in chemical signatures. Can this be used for COVID19 testing and monitoring? Any ideas? How soon one can develop testing tools using...

21 April 2020 6,257 3 View

Where can I find RAL 2012 (German standards Road regulations for secondary roads ) in the English version?

I found RAA available in english in the fgsv publisher website but could not find RAL , so please help me find it out

17 January 2019 118 0 View

Can curcumin flourescence be used for detection and quantification of curcumin in live and extracted samples?

LMSC imaging used in many samples for flurescence of live animal samples. Can we use this technique for detection and distribution of curcumin in live plant tissues?

19 August 2018 4,480 4 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Analysis of MHC-I and II alleles with CNVs and unassigned loci?

I am working on a dataset of MHC-I and II alleles from a bird species sequenced with Illumina. We were not able to assign alleles to loci through MHC-typer as we were over the limit of 150 alleles...

15 July 2024 182 1 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View