I wish to prepare peroxtesmo KO solution for studying stigma activity in plants. Kindly suggest how to prepare the solution.
Ajwain seeds are very small. The seed rate per ha is 2.5 kg for row sowing with seed hopper. The seed should also needs shallow sowing. Usually fine sand or rice semolina mixed with seed and sown....
04 September 2020 4,706 1 View
Turmeric is a polyploid. Mostly sterile. However, it sets seed occassionally. I have collected seed and kept in poretrays filled with cocopeat. I have not observed any germination even after 15...
28 August 2020 8,342 4 View
LMSC imaging used in many samples for flurescence of live animal samples. Can we use this technique for detection and distribution of curcumin in live plant tissues?
19 August 2018 4,316 4 View
Is there a standard ratio of concentration of monoclonal antibodies against a particular antigen for an antigen capture assay? To put it plainly how much monoclonal antibody is needed to bind to...
10 September 2014 7,365 8 View
We are planning to develop spectral signature library using hyper spectral remote sensing.
06 February 2014 5,658 6 View
The samples where - DNA isolation from liver, bacteria, cauliflower. The bacterial DNA sample are moving in the opposite direction to the liver and cauliflower samples.
19 December 2013 8,989 26 View
We are working on rainfall runoff modelling for Krishna river basin. We are interested to do the estimation of actual evapotranspiration from the remote sensing image using software.
13 September 2012 334 31 View
In 2016 I have developed a set of mutants in two varieties of turmeric-Mydukur and Prathibha. Now, in M1V4 evaluation, have identified a few are performing better than the parents. I am going to...
01 January 1970 4,130 4 View
I have observed, in India, in coastal humid tropics turmeric show sprouting around 2nd week of May which continues for another 45 days. Turmeric is usually harvested in this part during them...
01 January 1970 5,202 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...
23 February 2021 8,969 1 View
I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...
23 February 2021 9,007 3 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
22 February 2021 4,374 3 View
It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...
20 February 2021 5,634 3 View
i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...
18 February 2021 5,131 5 View
Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...
15 February 2021 8,462 3 View
Hello, I've got a plan to isolate mouse splenic Treg (WT and KO of target gene) and inject these CD45.2 Treg into tumor-bearing CD45.1 mice. My question is how many days do I need to wait to see...
11 February 2021 8,238 1 View