So far we have been using tissue tek provided by Leica to embed the tissue (Brain and Eyeball) while cryosectioning. I was told by a colleague that 15% PVP could be used as an alternative to it (as it will be cheaper and easily accessible). I would like to know if this may cause any kind of damage to the tissue while sectioning. I'm open to any suggestions/advice on the same.
Dear Vidya b Shukla,
admitting not to have worked ever using (histologically and/or -on smaller scale ultrastructurally) cryosectioning for brain and eyeballs I would like to point you to the possibility to find out by yourself using recent and older literature:
e.g. by using Scholar.Google with search phrase(s)
< PVP OR polyvinylpyrrolidone and CRYO AND EMBED > = [copy and paste the URL] https://scholar.google.com/scholar?q=PVP+OR+polyvinylpyrrolidone+and+CRYO+AND+EMBED&btnG=&hl=de&as_sdt=0%2C5 (860 results)
OR > PVP OR polyvinylpyrrolidone and CRYO OR FREEZ* AND EMBED < = https://scholar.google.com/scholar?q=PVP+OR+polyvinylpyrrolidone+and+CRYO+OR+FREEZ*+AND+EMBED&btnG=&hl=de&as_sdt=0%2C5 (894 results)
OR > PVP OR polyvinylpyrrolidone and CRYO OR FREEZE* AND EMBED < = https://scholar.google.com/scholar?hl=de&as_sdt=0,5&q=PVP+OR+polyvinylpyrrolidone+and+CRYO+OR+FREEZE*+AND+EMBED
Perhaps other colleagues having practical experience with the matter you describe will/can comment more in depth, but as I can see (and as I remember ancient as well as more recent cryo-techniques) PVP in different percentages can be / was added to specimens (as one example find:
Use of poly (vinylpyrrolidone) and poly (vinyl alcohol) for cryoultramicrotomy
KT Tokuyasu - The Histochemical journal, 1989 - Springer [http://link.springer.com/article/10.1007/BF01007491] )
Best wishes and regards, WM.
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