Can Octametylcyclotetrasiloxane (D4) be detected by HPLC? What detector normally used?

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How to prepare a perfusion buffer and calculate the concentrations of each enzyme?

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How to set up a PCR protocol?

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Can you connect an HPLC to a Mass Spec only at a certain time point?

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GC-MS retention index prediticon?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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Mário Silva

Hello.

Depending on your matrix, you can do it by UV detection. You can do it for sure with MS with +ESI ionization.

The trickiest part will be the chromatography itself, but you can find a few columns in market designed for this type of compounds.

Cheers.

Grzegorz Boczkaj

You can use also RID, corona CAD or ELSD.

Regards

Bruce Neagle

Dimethylsiloxane did not have a 'good' UV spectrum, but the answer depends on your sample matrix and the expected concentration.