Contemporary LGBTQ+ literature/writers in Ecuador?

I am looking to find contemporary (2005 and onward) LGBTQ+ literature/texts (novels, poems, digital literature, correspondence, social media writers) by Ecuadorian writers (preferably LGBTQ+ too,...

04 February 2020 9,780 3 View

What are the risk and resilience factors for student mental health?

Students in their final year of a Psychology degree at the Royal Holloway University London are researching factors affecting student mental health. All students studying in the UK please...

27 November 2019 241 2 View

Best experimental setup for ROS measurement after plant extract treatment using RAW 264.7 and H2DCFDA?

I am treating RAW 264.7 macrophages with some plant extracts dissolved in ethanol and measure if they decrease ROS/NO production. I have been having some trouble measuring ROS. A condensed...

20 August 2019 9,643 3 View

Why do no bands appear during glycoprotein staining?

Dear all, I'm trying to stain sugar groups attached to my protein with Glycoprotein staining (Thermo Scientific Pierce Glycoprotein Staining Kit #24562) after gel electrophoresis. I've added 4...

07 April 2019 3,224 2 View

How do I know that I can trust the chemical analysis results of surface and ground water quality samples from a subcontractor?

I was unable to perform the sampling and analysis for 40 surface water and 20 groundwater samples, so I hired an in-country consultant firm to do it. The results that they returned were difficult...

21 October 2018 8,937 10 View

Can anybody help me? Why is the genetic variance negative?

12 September 2018 1,959 14 View

Why can't we mate Thy-1 GFP to other transgenic lines?

We have a Thy-1 GFP mouse in our colony. We maintain the mouse as homozygous because the heterozygous animal did not show a strong enough signal. There doesn't appear to be any issues with the...

29 August 2018 3,576 4 View

What is the typical duration of a cell invasion assay? 24-48 hours, longer?

Can cell migration/invasion in the presence and absence of pharmacological inhibitors be tested for 1 week using this assay?

01 April 2018 5,885 5 View

Problems with protein purification by ion exchange chromatography

Hello everyone, I am trying to purify my protein (enzyme) expressed in Pichia pastoris through ion exchange chromatography. The protein is extracellular, so I extract the supernatant after...

27 July 2017 4,012 5 View

Ellipse on PCA analysis

Can I ask what the ellipse argument is used for in PCA? How to analysis the data by different ellipse shape and different direction in statistics? Is there any example for interpretation data by...

30 May 2017 7,219 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View